quartz flotation cell in San Francisco USA North America

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Apr 28, 2016· We processed 24cellsusing the original CEL-Seq protocol and 20cellsusing CEL-Seq2, as well ascellsloaded on the C1 (we had 72 singlecellscaptured, Additional file 3: Table S1). Spike-ins were added to eachcellallowing us to compare the efficiencies of transcript detection by fitting a linear relationship on the log-log plot (Fig. 2a ).

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The rocks are located on the coast of Sonoma County about 75 kmnorthofSan Francisco, within Sonoma Coast State Beach, a unit of the California State Park System. To date, I have located six sites consisting of one or more rubbing rocks, all within an area about 4 km in diameter. The two main sites are referred to as Mammoth Rocks and Jasper ...

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Apr 05, 2015·Cellsare harvested by washing once with 10 ml of PBS (add PBS to flask, agitate by hand 2–3 times and aspirate PBS off), then trypsinized with 4 ml of trypsin per T175 flask (incubatecellswith trypsin at 37 °C for 3–5 min, take offcellswith 6 ml of PBS by washing the walls of the flask and transferring to a 50 ml cornical tube).

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